› 5d58

X-ray diffraction
2.4Å resolution

In meso in situ serial X-ray crystallography structure of the PepTSt-Ala-Phe complex at 100 K

Released:
DOI: 10.2210/pdb5d58/pdb
PDB entry 5d58 coloured by chain, front view.
PDB entry 5d58 coloured by chain, side view.
PDB entry 5d58 coloured by chain, top view.
Source organism: Streptococcus thermophilus LMG 18311
Primary publication:
In meso in situ serial X-ray crystallography of soluble and membrane proteins at cryogenic temperatures.
Huang CY, Olieric V, Ma P, Howe N, Vogeley L, Liu X, Warshamanage R, Weinert T, Panepucci E, Kobilka B, Diederichs K, Wang M, Caffrey M
Acta Crystallogr D Struct Biol 72 93-112 (2016)
PMID: 26894538

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177587 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Major facilitator superfamily (MFS) profile domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 483 amino acids
Theoretical weight: 52.78 KDa
Source organism: Streptococcus thermophilus LMG 18311
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5M4H8 (Residues: 1-483; Coverage: 100%)
Gene names: dtpT, stu0970
Sequence domains: POT family
Structure domains: MFS general substrate transporter like domains

Ligands and Environments

5 bound ligands:
Ligand ALA 1 x ALA
Ligand PHE 1 x PHE
Ligand PO4 1 x PO4
Ligand 78M 21 x 78M
Ligand PE5 2 x PE5
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: C2221
Unit cell:
a: 100.16Å b: 109.52Å c: 111.47Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.209 0.251
Expression system: Escherichia coli

Quick links

Citations

3 review citations

G Protein-Coupled Receptors in Asthma Therapy: Pharmacology and Drug Action.
Wendell et al. (2020)
2 more

4 mentions without citation

Recent advances in understanding prodrug transport through the SLC15 family of proton-coupled transporters.
Minhas et al. (2020)
3 more