5d3u

X-ray diffraction
1.45Å resolution

Crystal structure of the 5-selective H176F mutant of Cytochrome TxtE

Released:
Source organism: Streptomyces scabiei 87.22
Entry authors: Cahn JKB, Dodani SC, Arnold FH

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative P450-like protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 427 amino acids
Theoretical weight: 47.14 KDa
Source organism: Streptomyces scabiei 87.22
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C9ZDC6 (Residues: 1-406; Coverage: 100%)
Gene name: SCAB_31831
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P212121
Unit cell:
a: 74.795Å b: 99.351Å c: 105.499Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.187 0.251
Expression system: Escherichia coli BL21(DE3)