5d33

X-ray diffraction
1.59Å resolution

Directed evolutionary changes in Kemp Eliminase KE07 - Crystal 12 round 7

Released:
Source organism: synthetic construct
Entry authors: Jackson CJ, Hong N-S, Carr PD

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
de novo kemp eliminase KE07 round 7 Chains: A, B
Molecule details ›
Chains: A, B
Length: 262 amino acids
Theoretical weight: 28.98 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)
Structure domains: Aldolase class I

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P212121
Unit cell:
a: 38.984Å b: 82.722Å c: 176.972Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.199 0.229
Expression system: Escherichia coli BL21(DE3)