PDBe 5d2l

X-ray diffraction
3.51Å resolution

Crystal structure of TCR C7 in complex with HCMV NLV epitope presented by HLA-A2

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
HLA class I histocompatibility antigen, A-2 alpha chain Chains: A, C, G, M
Molecule details ›
Chains: A, C, G, M
Length: 276 amino acids
Theoretical weight: 31.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01892 (Residues: 25-299; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, D, H, N
Molecule details ›
Chains: B, D, H, N
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
C7 TCR alpha chain Chains: E, I, K, O
Molecule details ›
Chains: E, I, K, O
Length: 205 amino acids
Theoretical weight: 22.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins
C7 TCR beta chain Chains: F, J, L, P
Molecule details ›
Chains: F, J, L, P
Length: 245 amino acids
Theoretical weight: 27.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins
65 kDa phosphoprotein Chains: Q, R, T, U
Molecule details ›
Chains: Q, R, T, U
Length: 9 amino acids
Theoretical weight: 943 Da
Source organism: Cytomegalovirus
Expression system: Not provided
UniProt:
  • Canonical: P18139 (Residues: 485-493; Coverage: 2%)
Gene name: UL83

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2221
Unit cell:
a: 151.76Å b: 366.643Å c: 151.948Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.274 0.27 0.355
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided