5d0g

X-ray diffraction
1.6Å resolution

Crystal structure of triple mutant (KDA to EGY) of adenylyl cyclase Ma1120 from Mycobacterium avium in complex with GTP and calcium ion

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-197681 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanylate cyclase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 177 amino acids
Theoretical weight: 19.62 KDa
Source organism: Mycobacterium avium subsp. avium 10-9275
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: V7LAR8 (Residues: 106-275; Coverage: 62%)
Gene name: O972_06080
Sequence domains: Adenylate and Guanylate cyclase catalytic domain
Structure domains: Nucleotide cyclase

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P21
Unit cell:
a: 52.07Å b: 55.63Å c: 55.14Å
α: 90° β: 108.67° γ: 90°
R-values:
R R work R free
0.19 0.189 0.214
Expression system: Escherichia coli BL21(DE3)