5d0a

X-ray diffraction
2.1Å resolution

Crystal structure of epoxyqueuosine reductase with cleaved RNA stem loop

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Epoxyqueuosine reductase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 437 amino acids
Theoretical weight: 48.74 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P97030 (Residues: 1-386; Coverage: 100%)
Gene names: BSU08910, queG, ygaP, yhbA
Sequence domains:
RNA (5'-R(*G*CP*AP*GP*AP*CP*UP*GP*UP*AP*AP*AP*UP*CP*UP*GP*C)-3') Chains: E, F
Molecule details ›
Chains: E, F
Length: 17 nucleotides
Theoretical weight: 5.43 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 90.587Å b: 111.118Å c: 96.008Å
α: 90° β: 104.9° γ: 90°
R-values:
R R work R free
0.186 0.184 0.225
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided