5czz

X-ray diffraction
2.6Å resolution

Crystal structure of Staphylococcus aureus Cas9 in complex with sgRNA and target DNA (TTGAAT PAM)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
CRISPR-associated endonuclease Cas9 Chain: A
Molecule details ›
Chain: A
Length: 1056 amino acids
Theoretical weight: 124.37 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: J7RUA5 (Residues: 1-1053; Coverage: 100%)
Gene name: cas9
Sequence domains:
DNA (28-MER) Chain: C
Molecule details ›
Chain: C
Length: 28 nucleotides
Theoretical weight: 8.47 KDa
Source organism: Staphylococcus aureus
Expression system: Not provided
DNA (5'-D(*TP*TP*GP*AP*AP*TP*AP*G)-3') Chain: D
Molecule details ›
Chain: D
Length: 8 nucleotides
Theoretical weight: 2.47 KDa
Source organism: Staphylococcus aureus
Expression system: Not provided
RNA (73-MER) Chain: B
Molecule details ›
Chain: B
Length: 73 nucleotides
Theoretical weight: 23.53 KDa
Source organism: Staphylococcus aureus
Expression system: Not provided

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21212
Unit cell:
a: 67.585Å b: 345.584Å c: 98.089Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.212 0.241
Expression systems:
  • Escherichia coli
  • Not provided