PDBe 5cxv

X-ray diffraction
2.7Å resolution

Structure of the human M1 muscarinic acetylcholine receptor bound to antagonist Tiotropium

Released:

Function and Biology Details

Reaction catalysed:
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Endolysin; Muscarinic acetylcholine receptor M1 Chain: A
Molecule details ›
Chain: A
Length: 515 amino acids
Theoretical weight: 58.65 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P11229 (Residues: 2-218, 227-227, 355-460; Coverage: 70%)
  • Canonical: P00720 (Residues: 2-11, 13-161; Coverage: 97%)
Gene names: CHRM1, E
Sequence domains:
FLAG peptide Chain: C
Molecule details ›
Chain: C
Length: 7 amino acids
Theoretical weight: 886 Da
Source organism: Escherichia virus T4
Expression system: Not provided

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P212121
Unit cell:
a: 58.053Å b: 72.186Å c: 175.688Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.231 0.282
Expression systems:
  • Spodoptera frugiperda
  • Not provided