5cws

X-ray diffraction
3.77Å resolution

Crystal structure of the intact Chaetomium thermophilum Nsp1-Nup49-Nup57 channel nucleoporin heterotrimer bound to its Nic96 nuclear pore complex attachment site

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
sAB-158 Fab Light Chain Chains: A, G
Molecule details ›
Chains: A, G
Length: 237 amino acids
Theoretical weight: 25.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
sAB-158 Fab Heavy Chain Chains: B, H
Molecule details ›
Chains: B, H
Length: 266 amino acids
Theoretical weight: 28.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Nucleoporin NSP1 Chains: C, I
Molecule details ›
Chains: C, I
Length: 208 amino acids
Theoretical weight: 23.56 KDa
Source organism: Chaetomium thermophilum var. thermophilum DSM 1495
Expression system: Escherichia coli
UniProt:
  • Canonical: G0SBQ3 (Residues: 467-674; Coverage: 31%)
Gene names: CTHT_0054390, NSP1
Nucleoporin NUP49 Chains: D, J
Molecule details ›
Chains: D, J
Length: 227 amino acids
Theoretical weight: 24.54 KDa
Source organism: Chaetomium thermophilum var. thermophilum DSM 1495
Expression system: Escherichia coli
UniProt:
  • Canonical: G0S4X2 (Residues: 244-244, 246-470; Coverage: 48%)
Gene names: CTHT_0031980, NUP49
Nucleoporin NUP57 Chains: E, K
Molecule details ›
Chains: E, K
Length: 247 amino acids
Theoretical weight: 28.79 KDa
Source organism: Chaetomium thermophilum var. thermophilum DSM 1495
Expression system: Escherichia coli
UniProt:
  • Canonical: G0S0R2 (Residues: 74-319; Coverage: 76%)
Gene names: CTHT_0010940, NUP57
Sequence domains:
Nucleoporin NIC96 Chains: F, L
Molecule details ›
Chains: F, L
Length: 74 amino acids
Theoretical weight: 7.79 KDa
Source organism: Chaetomium thermophilum var. thermophilum DSM 1495
Expression system: Escherichia coli
UniProt:
  • Canonical: G0S024 (Residues: 139-211; Coverage: 7%)
Gene names: CTHT_0008480, NIC96

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P212121
Unit cell:
a: 123.18Å b: 162.82Å c: 212.87Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.229 0.265
Expression system: Escherichia coli