› 5cws

X-ray diffraction
3.77Å resolution

Crystal structure of the intact Chaetomium thermophilum Nsp1-Nup49-Nup57 channel nucleoporin heterotrimer bound to its Nic96 nuclear pore complex attachment site

Released:
DOI: 10.2210/pdb5cws/pdb
PDB entry 5cws coloured by chain, front view.
PDB entry 5cws coloured by chain, side view.
PDB entry 5cws coloured by chain, top view.
Source organisms:
Primary publication:
Architecture of the fungal nuclear pore inner ring complex.
Stuwe T, Bley CJ, Thierbach K, Petrovic S, Schilbach S, Mayo DJ, Perriches T, Rundlet EJ, Jeon YE, Collins LN, Huber FM, Lin DH, Paduch M, Koide A, Lu V, Fischer J, Hurt E, Koide S, Kossiakoff AA, Hoelz A
Science 350 56-64 (2015)
PMID: 26316600

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-207866 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
sAB-158 Fab Light Chain Chains: A, G
Molecule details ›
Chains: A, G
Length: 237 amino acids
Theoretical weight: 25.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
sAB-158 Fab Heavy Chain Chains: B, H
Molecule details ›
Chains: B, H
Length: 266 amino acids
Theoretical weight: 28.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Nucleoporin NSP1 Chains: C, I
Molecule details ›
Chains: C, I
Length: 208 amino acids
Theoretical weight: 23.56 KDa
Source organism: Thermochaetoides thermophila DSM 1495
Expression system: Escherichia coli
UniProt:
  • Canonical: G0SBQ3 (Residues: 467-674; Coverage: 31%)
Gene names: CTHT_0054390, NSP1
Sequence domains: Nsp1-like C-terminal region
Nucleoporin NUP49 Chains: D, J
Molecule details ›
Chains: D, J
Length: 227 amino acids
Theoretical weight: 24.54 KDa
Source organism: Thermochaetoides thermophila DSM 1495
Expression system: Escherichia coli
UniProt:
  • Canonical: G0S4X2 (Residues: 244-244, 246-470; Coverage: 48%)
Gene names: CTHT_0031980, NUP49
Sequence domains: Nucleoporin Nup49 C-terminal helical region
Nucleoporin NUP57 Chains: E, K
Molecule details ›
Chains: E, K
Length: 247 amino acids
Theoretical weight: 28.79 KDa
Source organism: Thermochaetoides thermophila DSM 1495
Expression system: Escherichia coli
UniProt:
  • Canonical: G0S0R2 (Residues: 74-319; Coverage: 76%)
Gene names: CTHT_0010940, NUP57
Sequence domains:
Nucleoporin NIC96 Chains: F, L
Molecule details ›
Chains: F, L
Length: 74 amino acids
Theoretical weight: 7.79 KDa
Source organism: Thermochaetoides thermophila DSM 1495
Expression system: Escherichia coli
UniProt:
  • Canonical: G0S024 (Residues: 139-211; Coverage: 7%)
Gene names: CTHT_0008480, NIC96

Ligands and Environments

1 bound ligand:
Ligand OS 2 x OS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P212121
Unit cell:
a: 123.18Å b: 162.82Å c: 212.87Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.229 0.265
Expression system: Escherichia coli

Quick links

Citations

20 review citations

The Nuclear Pore Complex as a Flexible and Dynamic Gate.
Knockenhauer et al. (2016)
19 more

11 mentions without citation

The Structure Inventory of the Nuclear Pore Complex.
Schwartz TU. (2016)
10 more