› 5cw8

X-ray diffraction
2.6Å resolution

Crystal structure of Mycobacterium tuberculosis KstR in complex with 3-oxo-4-cholesten-26-oyl-CoA

Released:
DOI: 10.2210/pdb5cw8/pdb
PDB entry 5cw8 coloured by chain, front view.
PDB entry 5cw8 coloured by chain, side view.
PDB entry 5cw8 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
The Structure of the Transcriptional Repressor KstR in Complex with CoA Thioester Cholesterol Metabolites Sheds Light on the Regulation of Cholesterol Catabolism in Mycobacterium tuberculosis.
Ho NA, Dawes SS, Crowe AM, Casabon I, Gao C, Kendall SL, Baker EN, Eltis LD, Lott JS
J Biol Chem 291 7256-66 (2016)
PMID: 26858250

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161256 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH-type transcriptional repressor KstR Chains: A, B
Molecule details ›
Chains: A, B
Length: 203 amino acids
Theoretical weight: 22.35 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P96856 (Residues: 22-220; Coverage: 91%)
Gene names: Rv3574, kstR
Sequence domains:
Structure domains: Tetracycline Repressor, domain 2

Ligands and Environments

2 bound ligands:
Ligand 55X 2 x 55X
Ligand PGE 1 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Unit cell:
a: 49.694Å b: 67.095Å c: 124.231Å
α: 90° β: 96.74° γ: 90°
R-values:
R R work R free
0.196 0.194 0.236
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Cholesterol and fatty acids grease the wheels of Mycobacterium tuberculosis pathogenesis.
Wilburn et al. (2018)
4 more

1 mention without citation

The Structure of the Transcriptional Repressor KstR in Complex with CoA Thioester Cholesterol Metabolites Sheds Light on the Regulation of Cholesterol Catabolism in Mycobacterium tuberculosis.
Ho et al. (2016)