› 5crk

X-ray diffraction
2.48Å resolution

Crystal Structure of the MTERF1 F243A substitution bound to the termination sequence.

Released:
DOI: 10.2210/pdb5crk/pdb
PDB entry 5crk coloured by chain, front view.
PDB entry 5crk coloured by chain, side view.
PDB entry 5crk coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Base Flipping by MTERF1 Can Accommodate Multiple Conformations and Occurs in a Stepwise Fashion.
Byrnes J, Hauser K, Norona L, Mejia E, Simmerling C, Garcia-Diaz M
J Mol Biol 428 2542-2556 (2016)
PMID: 26523681

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118515 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transcription termination factor 1, mitochondrial Chain: O
Molecule details ›
Chain: O
Length: 324 amino acids
Theoretical weight: 37.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q99551 (Residues: 73-396; Coverage: 81%)
Gene names: MTERF, MTERF1
Sequence domains: mTERF
DNA (5'-D(*AP*TP*TP*AP*CP*CP*GP*GP*GP*CP*TP*CP*TP*GP*CP*CP*AP*TP*CP*TP*TP*A)-3') Chain: D
Molecule details ›
Chain: D
Length: 22 nucleotides
Theoretical weight: 6.68 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*TP*AP*AP*GP*AP*TP*GP*GP*CP*AP*GP*AP*GP*CP*CP*CP*GP*GP*TP*AP*AP*T)-3') Chain: E
Molecule details ›
Chain: E
Length: 22 nucleotides
Theoretical weight: 6.83 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2221
Unit cell:
a: 88.743Å b: 89.375Å c: 159.91Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.236 0.235 0.271
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

1 review citation

Regulation of a minimal transcriptome by repeat domain proteins.
Ferreira et al. (2018)
1 other article

1 mention without citation

Base Flipping by MTERF1 Can Accommodate Multiple Conformations and Occurs in a Stepwise Fashion.
Byrnes et al. (2016)