PDBe 5cp2

X-ray diffraction
2.36Å resolution

Human DNA polymerase lambda L431A mutant- Apoenzyme and complex with 6 paired DNA

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
monomeric
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase lambda Chains: A, B
Molecule details ›
Chains: A, B
Length: 334 amino acids
Theoretical weight: 37.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UGP5 (Residues: 242-575; Coverage: 58%)
Gene name: POLL
Sequence domains:
Structure domains:
DNA (5'-D(*CP*AP*GP*TP*AP*C)-3') Chain: P
Molecule details ›
Chain: P
Length: 6 nucleotides
Theoretical weight: 1.79 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(P*GP*TP*AP*CP*TP*G)-3') Chain: T
Molecule details ›
Chain: T
Length: 6 nucleotides
Theoretical weight: 1.82 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OTHER
Spacegroup: P6522
Unit cell:
a: 83.876Å b: 83.876Å c: 396.363Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.209 0.206 0.238
Expression systems:
  • Escherichia coli
  • Not provided