› 5ckr

X-ray diffraction
2.95Å resolution

Crystal Structure of MraY in complex with Muraymycin D2

Released:
DOI: 10.2210/pdb5ckr/pdb
PDB entry 5ckr coloured by chain, front view.
PDB entry 5ckr coloured by chain, side view.
PDB entry 5ckr coloured by chain, top view.
Source organism: Aquifex aeolicus VF5
Primary publication:
Structural insights into inhibition of lipid I production in bacterial cell wall synthesis.
Chung BC, Mashalidis EH, Tanino T, Kim M, Matsuda A, Hong J, Ichikawa S, Lee SY
Nature 533 557-560 (2016)
PMID: 27088606

Function and Biology Details

Reaction catalysed: 
UDP-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate = UMP + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130312 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phospho-N-acetylmuramoyl-pentapeptide-transferase Chain: A
Molecule details ›
Chain: A
Length: 365 amino acids
Theoretical weight: 40.95 KDa
Source organism: Aquifex aeolicus VF5
Expression system: Escherichia coli
UniProt:
  • Canonical: O66465 (Residues: 1-359; Coverage: 100%)
Gene names: aq_053, mraY
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand 57M 1 x 57M
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2221
Unit cell:
a: 94.48Å b: 102.05Å c: 135.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.248 0.247 0.261
Expression system: Escherichia coli

Quick links

Citations

12 review citations

The Membrane Steps of Bacterial Cell Wall Synthesis as Antibiotic Targets.
Liu et al. (2016)
11 more

16 mentions without citation

Structures of Bacterial MraY and Human GPT Provide Insights into Rational Antibiotic Design.
Mashalidis et al. (2020)
15 more