5cdp

X-ray diffraction
2.45Å resolution

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
DNA gyrase subunit A Chains: A, C
Molecule details ›
Chains: A, C
Length: 483 amino acids
Theoretical weight: 54.74 KDa
Source organism: Staphylococcus aureus subsp. aureus N315
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99XG5 (Residues: 9-491; Coverage: 54%)
Gene names: SA0006, gyrA
Sequence domains: DNA gyrase/topoisomerase IV, subunit A
DNA gyrase subunit B Chains: B, D
Molecule details ›
Chains: B, D
Length: 190 amino acids
Theoretical weight: 21.35 KDa
Source organism: Staphylococcus aureus subsp. aureus N315
Expression system: Escherichia coli
UniProt:
  • Canonical: P66937 (Residues: 417-543, 580-640; Coverage: 29%)
Gene names: SA0005, gyrB
Sequence domains: Toprim domain
DNA (5'-D(*AP*GP*CP*CP*GP*TP*AP*G*GP*GP*TP*AP*CP*CP*TP*AP*CP*GP*GP*CP*T)-3') Chains: E, F
Molecule details ›
Chains: E, F
Length: 8 nucleotides
Theoretical weight: 2.45 KDa
DNA (5'-D(*AP*GP*CP*CP*GP*TP*AP*G*GP*GP*TP*AP*CP*CP*TP*AP*CP*GP*GP*CP*T)-3') Chains: G, H
Molecule details ›
Chains: G, H
Length: 13 nucleotides
Theoretical weight: 3.97 KDa

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P61
Unit cell:
a: 93.364Å b: 93.364Å c: 411.244Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.178 0.177 0.211
Expression system: Escherichia coli