5cci

X-ray diffraction
4.1Å resolution

Structure of the Mg2+-bound synaptotagmin-1 SNARE complex (short unit cell form)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Vesicle-associated membrane protein 2 Chain: A
Molecule details ›
Chain: A
Length: 63 amino acids
Theoretical weight: 7.23 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63045 (Residues: 28-89; Coverage: 53%)
Gene names: Syb2, Vamp2
Syntaxin-1A Chain: B
Molecule details ›
Chain: B
Length: 67 amino acids
Theoretical weight: 7.84 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P32851 (Residues: 190-256; Coverage: 23%)
Gene names: Sap, Stx1a
Synaptosomal-associated protein 25 Chain: C
Molecule details ›
Chain: C
Length: 77 amino acids
Theoretical weight: 9.03 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P60881 (Residues: 7-83; Coverage: 37%)
  • Best match: P60881-2 (Residues: 7-83)
Gene names: Snap, Snap25
Synaptosomal-associated protein 25 Chain: D
Molecule details ›
Chain: D
Length: 65 amino acids
Theoretical weight: 7.47 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P60881 (Residues: 141-204; Coverage: 31%)
Gene names: Snap, Snap25
Synaptotagmin-1 Chains: E, F
Molecule details ›
Chains: E, F
Length: 281 amino acids
Theoretical weight: 32.25 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P21707 (Residues: 141-421; Coverage: 67%)
Gene name: Syt1
Sequence domains: C2 domain

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21212
Unit cell:
a: 69.064Å b: 171.759Å c: 146.561Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.279 0.276 0.323
Expression system: Escherichia coli BL21(DE3)