5c8b

X-ray diffraction
2.7Å resolution

Structural insights into the redesign of a sucrose phosphorylase by induced loop repositioning

Released:

Function and Biology Details

Reaction catalysed:
Sucrose + phosphate = D-fructose + alpha-D-glucose 1-phosphate
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sucrose phosphorylase Chain: B
Molecule details ›
Chain: B
Length: 514 amino acids
Theoretical weight: 57.55 KDa
Source organism: Bifidobacterium adolescentis
Expression system: Escherichia coli
UniProt:
  • Canonical: A0ZZH6 (Residues: 1-504; Coverage: 100%)
Gene names: BAD_0078, spl, sucP
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P43212
Unit cell:
a: 82.5Å b: 82.5Å c: 156.38Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.208 0.287
Expression system: Escherichia coli