› 5c7i

X-ray diffraction
2.01Å resolution

Mouse sperm Glyceraldehyde-3-phosphate dehydrogenase: apo enzyme

Released:
DOI: 10.2210/pdb5c7i/pdb
PDB entry 5c7i coloured by chain, front view.
PDB entry 5c7i coloured by chain, side view.
PDB entry 5c7i coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural analyses to identify selective inhibitors of glyceraldehyde 3-phosphate dehydrogenase-S, a sperm-specific glycolytic enzyme.
Danshina PV, Qu W, Temple BR, Rojas RJ, Miley MJ, Machius M, Betts L, O'Brien DA
Mol Hum Reprod 22 410-26 (2016)
PMID: 26921398

Function and Biology Details

Reaction catalysed: 
D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179137 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glyceraldehyde-3-phosphate dehydrogenase, testis-specific Chains: O, R
Molecule details ›
Chains: O, R
Length: 333 amino acids
Theoretical weight: 36.21 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q64467 (Residues: 107-439; Coverage: 76%)
Gene names: Gapd-s, Gapdhs, Gapds
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CSD 2 x CSD

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P3121
Unit cell:
a: 86.717Å b: 86.717Å c: 158.358Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.18 0.178 0.207
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The control of male fertility by spermatid-specific factors: searching for contraceptive targets from spermatozoon's head to tail.
Chen et al. (2016)
8 other articles

2 mentions without citation

Structural analyses to identify selective inhibitors of glyceraldehyde 3-phosphate dehydrogenase-S, a sperm-specific glycolytic enzyme.
Danshina et al. (2016)
1 more