PDBe 5c52

X-ray diffraction
3.64Å resolution

Probing the Structural and Molecular Basis of Nucleotide Selectivity by Human Mitochondrial DNA Polymerase gamma

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
DNA polymerase subunit gamma-1 Chain: A
Molecule details ›
Chain: A
Length: 1205 amino acids
Theoretical weight: 135.99 KDa
Source organism: Homo sapiens
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: P54098 (Residues: 25-1239; Coverage: 97%)
Gene names: MDP1, POLG, POLG1, POLGA
Sequence domains:
DNA polymerase subunit gamma-2, mitochondrial Chains: B, C
Molecule details ›
Chains: B, C
Length: 485 amino acids
Theoretical weight: 54.98 KDa
Source organism: Homo sapiens
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: Q9UHN1 (Residues: 1-485; Coverage: 100%)
Gene names: MTPOLB, POLG2
Sequence domains: Anticodon binding domain
Structure domains:
DNA (26-MER) Chain: T
Molecule details ›
Chain: T
Length: 26 nucleotides
Theoretical weight: 7.96 KDa
DNA (5'-D(*AP*AP*AP*AP*CP*GP*AP*GP*GP*GP*CP*CP*AP*GP*TP*GP*CP*CP*GP*TP*AP*C)-3') Chain: P
Molecule details ›
Chain: P
Length: 22 nucleotides
Theoretical weight: 6.78 KDa

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P41212
Unit cell:
a: 217.277Å b: 217.277Å c: 165.865Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.311 0.311 0.314
Expression system: Insect cell expression vector pTIE1