› 5byv

X-ray diffraction
2.16Å resolution

Crystal structure of MSM-13, a putative T1-like thiolase from Mycobacterium smegmatis

Released:
DOI: 10.2210/pdb5byv/pdb
PDB entry 5byv coloured by chain, front view.
PDB entry 5byv coloured by chain, side view.
PDB entry 5byv coloured by chain, top view.
Source organism: Mycolicibacterium smegmatis MC2 155
Primary publication:
Structural characterization of a mitochondrial 3-ketoacyl-CoA (T1)-like thiolase from Mycobacterium smegmatis.
Janardan N, Harijan RK, Kiema TR, Wierenga RK, Murthy MR
Acta Crystallogr D Biol Crystallogr 71 2479-93 (2015)
PMID: 26627655

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106329 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable acetyl-CoA acetyltransferase Chains: A, B, C, D, E, F, G, H, J, K, L, M
Molecule details ›
Chains: A, B, C, D, E, F, G, H, J, K, L, M
Length: 407 amino acids
Theoretical weight: 43.01 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Escherichia coli
UniProt:
  • Canonical: A0QUH3 (Residues: 1-407; Coverage: 100%)
Gene name: MSMEG_2207
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P61
Unit cell:
a: 190.959Å b: 190.959Å c: 264.681Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.171 0.169 0.2
Expression system: Escherichia coli

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Citations

1 review citation

Mutation update on ACAT1 variants associated with mitochondrial acetoacetyl-CoA thiolase (T2) deficiency.
Abdelkreem et al. (2019)