› 5buz

X-ray diffraction
3.1Å resolution

Crystal Structure of a Complex Between the SNARE Vam3 and the HOPS Vps33-Vps16 subcomplex from Chaetomium thermophilum

Released:
DOI: 10.2210/pdb5buz/pdb
PDB 5buz coloured by chain and viewed from the front.
PDB 5buz coloured by chain and viewed from the side.
PDB 5buz coloured by chain and viewed from the top.
Source organism: Thermochaetoides thermophila DSM 1495
Primary publication:
A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly.
Baker RW, Jeffrey PD, Zick M, Phillips BP, Wickner WT, Hughson FM
Science 349 1111-4 (2015)
PMID: 26339030

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
UBC core domain-containing protein Chains: A, D
Molecule details ›
Chains: A, D
Length: 669 amino acids
Theoretical weight: 73.99 KDa
Source organism: Thermochaetoides thermophila DSM 1495
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G0SCM5 (Residues: 139-806; Coverage: 83%)
Gene name: CTHT_0057760
Sequence domains: Sec1 family
Structure domains:
Probable vacuolar protein sorting-associated protein 16 homolog Chains: B, E
Molecule details ›
Chains: B, E
Length: 333 amino acids
Theoretical weight: 37.91 KDa
Source organism: Thermochaetoides thermophila DSM 1495
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G0S6M7 (Residues: 505-671, 673-816; Coverage: 38%)
Gene name: CTHT_0026760
Sequence domains: Vps16, C-terminal region
t-SNARE coiled-coil homology domain-containing protein Chains: C, F
Molecule details ›
Chains: C, F
Length: 67 amino acids
Theoretical weight: 7.5 KDa
Source organism: Thermochaetoides thermophila DSM 1495
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G0S236 (Residues: 181-245; Coverage: 24%)
Gene name: CTHT_0015850
Sequence domains: SNARE domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 95.039Å b: 95.95Å c: 164.06Å
α: 90° β: 99.12° γ: 90°
R-values:
R R work R free
0.196 0.194 0.233
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

32 review citations

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11 mentions without citation

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