› 5bus

X-ray diffraction
2.6Å resolution

O-succinylbenzoate Coenzyme A Synthetase (MenE) from Bacillus Subtilis, in complex with AMP

Released:
DOI: 10.2210/pdb5bus/pdb
PDB entry 5bus coloured by chain, front view.
PDB entry 5bus coloured by chain, side view.
PDB entry 5bus coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Primary publication:
Structural Basis for the ATP-dependent Configuration of Adenylation Active Site in Bacillus subtilis o-Succinylbenzoyl-CoA Synthetase.
Chen Y, Sun Y, Song H, Guo Z
J Biol Chem 290 23971-83 (2015)
PMID: 26276389

Function and Biology Details

Reaction catalysed: 
ATP + 2-succinylbenzoate + CoA = AMP + diphosphate + 2-succinylbenzoyl-CoA
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150139 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-succinylbenzoate--CoA ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 494 amino acids
Theoretical weight: 55.27 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P23971 (Residues: 1-486; Coverage: 100%)
Gene names: BSU30790, menE
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CL 4 x CL
Ligand AMP 2 x AMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P43
Unit cell:
a: 121.811Å b: 121.811Å c: 98.013Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.19 0.234
Expression system: Escherichia coli BL21

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Citations

7 citation in other articles

Discovery of a Diazo-Forming Enzyme in Cremeomycin Biosynthesis.
Waldman et al. (2018)
6 more

4 mentions without citation

BioSM: metabolomics tool for identifying endogenous mammalian biochemical structures in chemical structure space.
Hamdalla et al. (2013)
3 more