› 5bsg

X-ray diffraction
1.95Å resolution

Crystal structure of Medicago truncatula (delta)1-Pyrroline-5-Carboxylate Reductase (MtP5CR) in complex with NADP+

Released:
DOI: 10.2210/pdb5bsg/pdb
PDB entry 5bsg coloured by chain, front view.
PDB entry 5bsg coloured by chain, side view.
PDB entry 5bsg coloured by chain, top view.
Source organism: Medicago truncatula
Primary publication:
The structure of Medicago truncatula δ(1)-pyrroline-5-carboxylate reductase provides new insights into regulation of proline biosynthesis in plants.
Ruszkowski M, Nocek B, Forlani G, Dauter Z
Front Plant Sci 6 869 (2015)
PMID: 26579138

Function and Biology Details

Reaction catalysed: 
L-proline + NAD(P)(+) = 1-pyrroline-5-carboxylate + NAD(P)H
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124580 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyrroline-5-carboxylate reductase Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 277 amino acids
Theoretical weight: 28.79 KDa
Source organism: Medicago truncatula
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G7KRM5 (Residues: 1-274; Coverage: 100%)
Gene names: 11414041, MTR_7g090160, MtrunA17_Chr7g0256431
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAP 10 x NAP
2 bound ligands:
Ligand MPO 10 x MPO
Ligand CL 10 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P1
Unit cell:
a: 87.239Å b: 100.3Å c: 100.786Å
α: 68.13° β: 85.76° γ: 89.3°
R-values:
R R work R free
0.156 0.155 0.178
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Structure, biochemistry, and gene expression patterns of the proline biosynthetic enzyme pyrroline-5-carboxylate reductase (PYCR), an emerging cancer therapy target.
Bogner et al. (2021)
2 more

2 mentions without citation

The structure of Medicago truncatula δ(1)-pyrroline-5-carboxylate reductase provides new insights into regulation of proline biosynthesis in plants.
Ruszkowski et al. (2015)
1 more