X-ray diffraction
1.89Å resolution

Crystal structure of K66A mutant of human macrophage migration inhibitory factor


Function and Biology Details

Reactions catalysed:
L-dopachrome = 5,6-dihydroxyindole-2-carboxylate
Keto-phenylpyruvate = enol-phenylpyruvate
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macrophage migration inhibitory factor Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 114 amino acids
Theoretical weight: 12.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: P14174 (Residues: 2-115; Coverage: 99%)
Gene names: GLIF, MIF, MMIF
Sequence domains: Macrophage migration inhibitory factor (MIF)
Structure domains: Macrophage Migration Inhibitory Factor

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 67.275Å b: 67.771Å c: 87.535Å
α: 90° β: 90° γ: 90°
R R work R free
0.199 0.198 0.218
Expression system: Escherichia coli