5brm

X-ray diffraction
2.65Å resolution

Structural basis for Mob1-dependent activation of the core Mst-Lats kinase cascade in Hippo signaling

Released:

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero pentadecamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
MOB kinase activator 1A Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 177 amino acids
Theoretical weight: 20.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9H8S9 (Residues: 41-216; Coverage: 82%)
Gene names: C2orf6, MOB1A, MOB4B, MOBK1B, MOBKL1B
Structure domains: MOB kinase activator
Serine/threonine-protein kinase 3 20kDa subunit Chains: G, H, I, J, K, L, M, N, O
Molecule details ›
Chains: G, H, I, J, K, L, M, N, O
Length: 31 amino acids
Theoretical weight: 3.78 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q13188 (Residues: 371-401; Coverage: 6%)
Gene names: KRS1, MST2, STK3

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P3121
Unit cell:
a: 142.188Å b: 142.188Å c: 135.981Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.245 0.244 0.268
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided