› 5br5

X-ray diffraction
2Å resolution

Structure of bacteriorhodopsin crystallized from ND-MSP1E3D1

Released:
DOI: 10.2210/pdb5br5/pdb
PDB entry 5br5 coloured by chain, front view.
PDB entry 5br5 coloured by chain, side view.
PDB entry 5br5 coloured by chain, top view.
Source organism: Halobacterium salinarum
Primary publication:
Integral Membrane Proteins Can Be Crystallized Directly from Nanodiscs
Nikolaev M, Round E, Gushchin I, Polovinkin V, Balandin T, Kuzmichev P, Shevchenko V, Borshchevskiy V, Kuklin A, Round A, Bernhard F, Willbold D, Buldt G, Gordeliy V
Cryst Growth Des 17 945-948 (2017)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136413 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacteriorhodopsin Chain: A
Molecule details ›
Chain: A
Length: 249 amino acids
Theoretical weight: 26.93 KDa
Source organism: Halobacterium salinarum
Expression system: Halobacterium salinarum
UniProt:
  • Canonical: P02945 (Residues: 14-262; Coverage: 95%)
Gene names: VNG_1467G, bop
Sequence domains: Bacteriorhodopsin-like protein
Structure domains: Rhodopsin 7-helix transmembrane proteins

Ligands and Environments

1 bound ligand:
Ligand RET 1 x RET
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P63
Unit cell:
a: 60.98Å b: 60.98Å c: 109.99Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.207 0.205 0.249
Expression system: Halobacterium salinarum

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