PDBe 5bo0

X-ray diffraction
2.91Å resolution

Crystal structure of Human MCM2 HBD and ASF1b chaperoning a histone H3.2-H4 dimer

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Histone H3.2 Chain: A
Molecule details ›
Chain: A
Length: 80 amino acids
Theoretical weight: 9.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q71DI3 (Residues: 57-136; Coverage: 59%)
Gene names: H3F2, H3FM, HIST2H3A, HIST2H3C, HIST2H3D
Structure domains: Histone, subunit A
Histone H4 Chain: B
Molecule details ›
Chain: B
Length: 102 amino acids
Theoretical weight: 11.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62805 (Residues: 2-103; Coverage: 99%)
Gene names: H4/A, H4/B, H4/C, H4/D, H4/E, H4/G, H4/H, H4/I, H4/J, H4/K, H4/M, H4/N, H4/O, H4F2, H4FA, H4FB, H4FC, H4FD, H4FE, H4FG, H4FH, H4FI, H4FJ, H4FK, H4FM, H4FN, H4FO, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4A, HIST2H4B, HIST4H4
Sequence domains: Centromere kinetochore component CENP-T histone fold
Structure domains: Histone, subunit A
DNA replication licensing factor MCM2 Chain: C
Molecule details ›
Chain: C
Length: 70 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P49736 (Residues: 61-130; Coverage: 8%)
Gene names: BM28, CCNL1, CDCL1, KIAA0030, MCM2
Histone chaperone ASF1B Chain: D
Molecule details ›
Chain: D
Length: 158 amino acids
Theoretical weight: 17.92 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NVP2 (Residues: 1-158; Coverage: 78%)
Gene name: ASF1B
Sequence domains: ASF1 like histone chaperone
Structure domains: Histone chaperone ASF1-like

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P3121
Unit cell:
a: 112.964Å b: 112.964Å c: 98.226Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.193 0.19 0.238
Expression system: Escherichia coli