› 5bjz

X-ray diffraction
1.95Å resolution

Crystal structure of maltose binding protein in complex with an allosteric synthetic antibody

Released:
DOI: 10.2210/pdb5bjz/pdb
PDB entry 5bjz coloured by chain, front view.
PDB entry 5bjz coloured by chain, side view.
PDB entry 5bjz coloured by chain, top view.
Source organisms:
Primary publication:
Engineered synthetic antibodies as probes to quantify the energetic contributions of ligand binding to conformational changes in proteins.
Mukherjee S, Griffin DH, Horn JR, Rizk SS, Nocula-Lugowska M, Malmqvist M, Kim SS, Kossiakoff AA
J Biol Chem 293 2815-2828 (2018)
PMID: 29321208

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-229873 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Maltose/maltodextrin-binding periplasmic protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 398 amino acids
Theoretical weight: 44.18 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEX9 (Residues: 26-392; Coverage: 99%)
Gene names: JW3994, b4034, malE
Sequence domains: Bacterial extracellular solute-binding protein
Synthetic antibody, Fab fragment, Heavy Chain Chains: C, D
Molecule details ›
Chains: C, D
Length: 238 amino acids
Theoretical weight: 25.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Synthetic antibody, Fab fragment, Light Chain Chains: H, L
Molecule details ›
Chains: H, L
Length: 216 amino acids
Theoretical weight: 23.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
3 bound ligands:
Ligand GOL 5 x GOL
Ligand EPE 1 x EPE
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 98.39Å b: 87.59Å c: 114.65Å
α: 90° β: 114.29° γ: 90°
R-values:
R R work R free
0.196 0.194 0.229
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Surface Plasmon Resonance for Biomarker Detection: Advances in Non-invasive Cancer Diagnosis.
Bellassai et al. (2019)
2 more

3 mentions without citation

Synthetic single domain antibodies for the conformational trapping of membrane proteins.
Zimmermann et al. (2018)
2 more