› 5b7s

X-ray diffraction
2.58Å resolution

Apo structure of Cysteine Desulfurase from Thermococcus onnurineus NA1

Released:
DOI: 10.2210/pdb5b7s/pdb
PDB entry 5b7s coloured by chain, front view.
PDB entry 5b7s coloured by chain, side view.
PDB entry 5b7s coloured by chain, top view.
Source organism: Thermococcus onnurineus NA1
Primary publication:
Catalytic Intermediate Crystal Structures of Cysteine Desulfurase from the Archaeon Thermococcus onnurineus NA1.
Ho TH, Huynh KH, Nguyen DQ, Park H, Jung K, Sur B, Ahn YJ, Cha SS, Kang LW
Archaea 2017 5395293 (2017)
PMID: 28536498

Function and Biology Details

Reaction catalysed: 
(1a) L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110406 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cysteine desulfurase Chains: A, B
Molecule details ›
Chains: A, B
Length: 419 amino acids
Theoretical weight: 46.9 KDa
Source organism: Thermococcus onnurineus NA1
Expression system: Escherichia coli
UniProt:
  • Canonical: B6YT87 (Residues: 1-399; Coverage: 100%)
Gene name: TON_0289
Sequence domains: Aminotransferase class-V

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
1 modified residue:
Ligand LLP 2 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P212121
Unit cell:
a: 67.006Å b: 92.527Å c: 145.444Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.203 0.295
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Biocatalytic synthesis of non-standard amino acids by a decarboxylative aldol reaction.
Ellis et al. (2022)

2 mentions without citation

Structural evidence for a latch mechanism regulating access to the active site of SufS-family cysteine desulfurases.
Dunkle et al. (2020)
1 more