5b74

X-ray diffraction
2.04Å resolution

Crystal structure of conjoined Pyrococcus furiosus L-asparaginase with peptide

Released:
Entry authors: Sharma P, Tomar R, Nath SK, Kundu B, Ashish

Function and Biology Details

Reaction catalysed:
L-asparagine + H(2)O = L-aspartate + NH(3)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
L-asparaginase Chain: A
Molecule details ›
Chain: A
Length: 182 amino acids
Theoretical weight: 19.89 KDa
Source organism: Pyrococcus furiosus DSM 3638
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8TZE8 (Residues: 1-182; Coverage: 56%)
Gene name: PF2047
Structure domains: L-asparaginase, N-terminal domain
L-asparaginase Chain: B
Molecule details ›
Chain: B
Length: 127 amino acids
Theoretical weight: 13.9 KDa
Source organism: Pyrococcus furiosus DSM 3638
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8TZE8 (Residues: 202-326; Coverage: 38%)
Gene name: PF2047
Structure domains: Rossmann fold
LEU-VAL-VAL-ASN Chain: D
Molecule details ›
Chain: D
Length: 4 amino acids
Theoretical weight: 444 Da
Source organism: Pyrococcus furiosus
Expression system: Not provided

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P6522
Unit cell:
a: 92.01Å b: 92.01Å c: 187.081Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.214 0.213 0.242
Expression systems:
  • Escherichia coli
  • Not provided