5b5u

X-ray diffraction
2.61Å resolution

Crystal structure of truncated Pyrococcus furiosus L-asparaginase with peptide

Released:
Entry authors: Sharma P, Tomar R, Nath SK, Kundu B, Ashish

Function and Biology Details

Reaction catalysed:
L-asparagine + H(2)O = L-aspartate + NH(3)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
L-asparaginase Chain: A
Molecule details ›
Chain: A
Length: 178 amino acids
Theoretical weight: 19.47 KDa
Source organism: Pyrococcus furiosus DSM 3638
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8TZE8 (Residues: 1-178; Coverage: 55%)
Gene name: PF2047
Structure domains: L-asparaginase, N-terminal domain
L-asparaginase Chain: B
Molecule details ›
Chain: B
Length: 127 amino acids
Theoretical weight: 13.9 KDa
Source organism: Pyrococcus furiosus DSM 3638
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8TZE8 (Residues: 202-326; Coverage: 38%)
Gene name: PF2047
Structure domains: Rossmann fold
LEU-VAL-VAL-ASN Chains: C, E
Molecule details ›
Chains: C, E
Length: 4 amino acids
Theoretical weight: 444 Da
Source organism: Pyrococcus furiosus
Expression system: Not provided

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P6522
Unit cell:
a: 91.31Å b: 91.31Å c: 186.971Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.205 0.203 0.242
Expression systems:
  • Escherichia coli
  • Not provided