› 5b5g

X-ray diffraction
1.5Å resolution

Crystal structure of ALiS4-Streptavidin complex

Released:
DOI: 10.2210/pdb5b5g/pdb
PDB entry 5b5g coloured by chain, front view.
PDB entry 5b5g coloured by chain, side view.
PDB entry 5b5g coloured by chain, top view.
Source organism: Streptomyces avidinii
Primary publication:
Improving the Solubility of Artificial Ligands of Streptavidin to Enable More Practical Reversible Switching of Protein Localization in Cells.
Tachibana R, Terai T, Boncompain G, Sugiyama S, Saito N, Perez F, Urano Y
Chembiochem 18 358-362 (2017)
PMID: 27905160

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptavidin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 120 amino acids
Theoretical weight: 12.69 KDa
Source organism: Streptomyces avidinii
UniProt:
  • Canonical: P22629 (Residues: 40-159; Coverage: 76%)
Sequence domains: Avidin family
Structure domains: Avidin-like

Ligands and Environments

2 bound ligands:
Ligand SO3 7 x SO3
Ligand 6FX 2 x 6FX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P21
Unit cell:
a: 57.921Å b: 84.423Å c: 46.138Å
α: 90° β: 98.98° γ: 90°
R-values:
R R work R free
0.147 0.145 0.193

Quick links

Citations

1 review citation

Genetically encoded fluorescent tools: Shining a little light on ER-to-Golgi transport.
Seiler et al. (2022)