5b57

X-ray diffraction
2.8Å resolution

Inward-facing conformation of ABC heme importer BhuUV from Burkholderia cenocepacia

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-110055 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Hemin ABC transport system, membrane protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 385 amino acids
Theoretical weight: 38.8 KDa
Source organism: Burkholderia cenocepacia J2315
Expression system: Escherichia coli
UniProt:
  • Canonical: B4EKB4 (Residues: 1-362; Coverage: 100%)
Gene names: BCAM2629, hmuU
Sequence domains: FecCD transport family
Structure domains: ABC transporter involved in vitamin B12 uptake, BtuC
ABC transporter domain-containing protein Chains: C, D
Molecule details ›
Chains: C, D
Length: 273 amino acids
Theoretical weight: 29.23 KDa
Source organism: Burkholderia cenocepacia J2315
Expression system: Escherichia coli
UniProt:
  • Canonical: B4EKB5 (Residues: 1-273; Coverage: 100%)
Gene names: BCAM2630, hmuV
Sequence domains: ABC transporter
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: P212121
Unit cell:
a: 110.824Å b: 118.283Å c: 142.747Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.212 0.265
Expression system: Escherichia coli