5b4t

X-ray diffraction
1.19Å resolution

Crystal structure of D-3-hydroxybutyrate dehydrogenase from Alcaligenes faecalis complexed with NAD+ and a substrate D-3-hydroxybutyrate

Released:

Function and Biology Details

Reaction catalysed:
(R)-3-hydroxybutanoate + NAD(+) = acetoacetate + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-hydroxybutyrate dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 260 amino acids
Theoretical weight: 27.12 KDa
Source organism: Alcaligenes faecalis
Expression system: Escherichia coli
UniProt:
  • Canonical: D0VWQ0 (Residues: 1-260; Coverage: 100%)
Gene names: DF183_01165, EGY22_17825
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAD 1 x NAD
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P42212
Unit cell:
a: 62.78Å b: 62.78Å c: 119.49Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.158 0.157 0.173
Expression system: Escherichia coli