5b3f

X-ray diffraction
2.5Å resolution

Crystal structure of phosphoribulokinase from Methanospirillum hungatei

Released:

Function and Biology Details

Reaction catalysed:
ATP + D-ribulose 5-phosphate = ADP + D-ribulose 1,5-bisphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoribulokinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 343 amino acids
Theoretical weight: 39.68 KDa
Source organism: Methanospirillum hungatei JF-1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2FUB5 (Residues: 1-323; Coverage: 100%)
Gene name: Mhun_0794
Sequence domains: Phosphoribulokinase / Uridine kinase family

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P212121
Unit cell:
a: 78.414Å b: 93.822Å c: 99.942Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.223 0.279
Expression system: Escherichia coli