5ayr

X-ray diffraction
2.4Å resolution

The crystal structure of SAUGI/human UDG complex

Released:

Function and Biology Details

Reaction catalysed:
Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Uracil-DNA glycosylase Chains: A, C
Molecule details ›
Chains: A, C
Length: 231 amino acids
Theoretical weight: 26.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P13051 (Residues: 94-313; Coverage: 70%)
Gene names: DGU, UNG, UNG1, UNG15
Sequence domains: Uracil DNA glycosylase superfamily
Structure domains: Uracil-DNA glycosylase-like domain
Uncharacterized protein Chains: B, D
Molecule details ›
Chains: B, D
Length: 112 amino acids
Theoretical weight: 13.37 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q936H5 (Residues: 1-112; Coverage: 100%)
Sequence domains: S. aureus uracil DNA glycosylase inhibitor
Structure domains: S. aureus uracil DNA glycosylase inhibitor

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: C2
Unit cell:
a: 177.831Å b: 52.853Å c: 82.338Å
α: 90° β: 112.36° γ: 90°
R-values:
R R work R free
0.167 0.165 0.198
Expression system: Escherichia coli