› 5aoz

X-ray diffraction
1.14Å resolution

High resolution SeMet structure of the third cohesin from Ruminococcus flavefaciens scaffoldin protein, ScaB

Released:
DOI: 10.2210/pdb5aoz/pdb
PDB entry 5aoz coloured by chain, front view.
PDB entry 5aoz coloured by chain, side view.
PDB entry 5aoz coloured by chain, top view.
Source organism: Ruminococcus flavefaciens FD-1
Entry authors: Bule P, Carvalho AL, Santos H, Fontes CMGA, Najmudin S

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106042 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cohesin domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 169 amino acids
Theoretical weight: 18.27 KDa
Source organism: Ruminococcus flavefaciens FD-1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0AEF4 (Residues: 399-544; Coverage: 7%)
Gene name: scaB
Structure domains: Immunoglobulin-like

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P41212
Unit cell:
a: 60.427Å b: 60.427Å c: 86.509Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.12 0.118 0.142
Expression system: Escherichia coli BL21(DE3)

Quick links