› 5ajg

X-ray diffraction
1.11Å resolution

Structure of Infrared Fluorescent Protein IFP1.4 AT 1.11 Angstrom resolution

Released:
DOI: 10.2210/pdb5ajg/pdb
PDB entry 5ajg coloured by chain, front view.
PDB entry 5ajg coloured by chain, side view.
PDB entry 5ajg coloured by chain, top view.
Source organism: Deinococcus radiodurans
Primary publication:
Structural Determinants of Improved Fluorescence in a Family of Bacteriophytochrome-Based Infrared Fluorescent Proteins: Insights from Continuum Electrostatic Calculations and Molecular Dynamics Simulations.
Feliks M, Lafaye C, Shu X, Royant A, Field M
Biochemistry 55 4263-74 (2016)
PMID: 27471775

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193366 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacteriophytochrome Chain: A
Molecule details ›
Chain: A
Length: 329 amino acids
Theoretical weight: 35.96 KDa
Source organism: Deinococcus radiodurans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9RZA4 (Residues: 1-321; Coverage: 43%)
Gene names: DR_A0050, bphP
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand LBV 1 x LBV
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2
Unit cell:
a: 95.361Å b: 53.028Å c: 66.219Å
α: 90° β: 90.9° γ: 90°
R-values:
R R work R free
0.147 0.146 0.168
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 citation in other articles

The Origin of Ultrafast Multiphasic Dynamics in Photoisomerization of Bacteriophytochrome.
Wang et al. (2020)
11 more