Function and Biology Details
Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
- not assigned
Biological process:
Cellular component:
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
General negative regulator of transcription (preferred)
PDBe Complex ID:
PDB-CPX-150544 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
General negative regulator of transcription subunit 1
Molecule details ›
Chains: A, C, E, G, I, K
Length: 556 amino acids
Theoretical weight: 64.81 KDa
Source organism: Saccharomyces cerevisiae CEN.PK113-7D
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: CCR4-Not complex component, Not1
Structure domains:
Length: 556 amino acids
Theoretical weight: 64.81 KDa
Source organism: Saccharomyces cerevisiae CEN.PK113-7D
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
P25655 (Residues: 1541-2093; Coverage: 26%)
Sequence domains: CCR4-Not complex component, Not1
Structure domains:
General negative regulator of transcription subunit 4
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
SLS BEAMLINE X10SA
Spacegroup:
P3221
Expression system: Escherichia coli BL21(DE3)
