› 5ahy

X-ray diffraction
2.15Å resolution

Halorhodopsin from Halobacterium salinarum in a new rhombohedral crystal form

Released:
DOI: 10.2210/pdb5ahy/pdb
PDB entry 5ahy coloured by chain, front view.
PDB entry 5ahy coloured by chain, side view.
PDB entry 5ahy coloured by chain, top view.
Source organism: Halobacterium salinarum
Primary publication:
Structure of Halorhodopsin from Halobacterium salinarum in a new crystal form that imposes little restraint on the E-F loop.
Schreiner M, Schlesinger R, Heberle J, Niemann HH
J Struct Biol 190 373-8 (2015)
PMID: 25916754

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109190 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Halorhodopsin Chain: A
Molecule details ›
Chain: A
Length: 261 amino acids
Theoretical weight: 27.95 KDa
Source organism: Halobacterium salinarum
Expression system: Halobacterium salinarum
UniProt:
  • Canonical: B0R2U4 (Residues: 20-274; Coverage: 93%)
Gene names: OE_1299R, hop
Sequence domains: Bacteriorhodopsin-like protein
Structure domains: Rhodopsin 7-helix transmembrane proteins

Ligands and Environments

3 bound ligands:
Ligand RET 1 x RET
Ligand CL 2 x CL
Ligand BOG 1 x BOG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: R32
Unit cell:
a: 103.651Å b: 103.651Å c: 138.588Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.187 0.185 0.227
Expression system: Halobacterium salinarum

Quick links

Citations

1 review citation

Diversity, Mechanism, and Optogenetic Application of Light-Driven Ion Pump Rhodopsins.
Inoue K. (2021)
4 other articles

4 mentions without citation

The crystal structures of a chloride-pumping microbial rhodopsin and its proton-pumping mutant illuminate proton transfer determinants.
Besaw et al. (2020)
3 more