5ags

X-ray diffraction
1.47Å resolution

Crystal structure of the LeuRS editing domain of Mycobacterium tuberculosis in complex with the adduct 3-(Aminomethyl)-4-bromo-7-ethoxybenzo[c][1,2]oxaborol-1(3H)-ol-AMP

Released:

Function and Biology Details

Reaction catalysed:
ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Leucine--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 232 amino acids
Theoretical weight: 24.89 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P9WFV1 (Residues: 309-513; Coverage: null%)
Gene names: MTCY21D4.04, Rv0041, leuS
Structure domains: Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 44.75Å b: 62.16Å c: 76.99Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.183 0.206
Expression system: Escherichia coli K-12