5ae0

X-ray diffraction
1.04Å resolution

Perdeuterated mouse CNPase catalytic domain at atomic resolution

Released:

Function and Biology Details

Reaction catalysed:
Nucleoside 2',3'-cyclic phosphate + H(2)O = nucleoside 2'-phosphate
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2',3'-cyclic-nucleotide 3'-phosphodiesterase Chain: A
Molecule details ›
Chain: A
Length: 221 amino acids
Theoretical weight: 24.29 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P16330 (Residues: 179-398; Coverage: 52%)
Gene names: Cnp, Cnp1
Structure domains: 2',3'-cyclic nucleotide 3'-phosphodiesterase superfamily

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-3
Spacegroup: P21
Unit cell:
a: 39.65Å b: 47.56Å c: 51.22Å
α: 90° β: 97.51° γ: 90°
R-values:
R R work R free
0.128 0.127 0.156
Expression system: Escherichia coli BL21(DE3)