5ac9

Electron Microscopy
3.2Å resolution

Structure-based energetics of protein interfaces guide Foot-and-Mouth disease virus vaccine design

Released:

Function and Biology Details

Reactions catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
NTP + H(2)O = NDP + phosphate
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Autocatalytically cleaves itself from the polyprotein of the foot-and-mouth disease virus by hydrolysis of a Lys-|-Gly bond, but then cleaves host cell initiation factor eIF-4G at bonds -Gly-|-Arg- and -Lys-|-Arg-.
Biochemical function:
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assemblies composition:
Non-polymer only 240-mer (preferred)
Non-polymer only tetramer
Non-polymer only icosamer
Non-polymer only 24-mer
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Protein 3B-1 Chain: 1
Molecule details ›
Chain: 1
Length: 208 amino acids
Theoretical weight: 22.87 KDa
Source organism: Foot-and-mouth disease virus - type O
Expression system: Mesocricetus auratus
UniProt:
  • Canonical: Q6PMW3 (Residues: 725-932; Coverage: 9%)
Structure domains: Jelly Rolls
Protein 3B-1 Chain: 2
Molecule details ›
Chain: 2
Length: 218 amino acids
Theoretical weight: 24.42 KDa
Source organism: Foot-and-mouth disease virus - type O
Expression system: Mesocricetus auratus
UniProt:
  • Canonical: Q6PMW3 (Residues: 287-504; Coverage: 9%)
Sequence domains: picornavirus capsid protein
Structure domains: Jelly Rolls
Protein 3B-1 Chain: 3
Molecule details ›
Chain: 3
Length: 220 amino acids
Theoretical weight: 23.93 KDa
Source organism: Foot-and-mouth disease virus - type O
Expression system: Mesocricetus auratus
UniProt:
  • Canonical: Q6PMW3 (Residues: 505-724; Coverage: 9%)
Sequence domains: picornavirus capsid protein
Structure domains: Jelly Rolls
Protein 3B-1 Chain: 4
Molecule details ›
Chain: 4
Length: 85 amino acids
Theoretical weight: 8.77 KDa
Source organism: Foot-and-mouth disease virus - type O
Expression system: Mesocricetus auratus
UniProt:
  • Canonical: Q6PMW3 (Residues: 202-286; Coverage: 4%)
Sequence domains: Viral protein VP4 subunit
Structure domains: Capsid protein VP4 superfamily, Picornavirus

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-3129
Expression system: Mesocricetus auratus