X-ray diffraction
1.65Å resolution

S.enterica HisA mutant D7N, G102A, V106M, D176A

Source organism: Salmonella enterica
Entry authors: Guo X, Soderholm A, Newton M, Nasvall J, Andersson D, Patrick W, Selmer M

Function and Biology Details

Reaction catalysed:
1-(5-phospho-beta-D-ribosyl)-5-((5-phospho-beta-D-ribosylamino)methylideneamino)imidazole-4-carboxamide = 5-((5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino)-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase Chain: A
Molecule details ›
Chain: A
Length: 253 amino acids
Theoretical weight: 27.13 KDa
Source organism: Salmonella enterica
Expression system: Escherichia coli BL21
  • Canonical: P10372 (Residues: 1-245; Coverage: 100%)
Gene names: STM2076, hisA
Sequence domains: Histidine biosynthesis protein
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P3121
Unit cell:
a: 46.555Å b: 46.555Å c: 196.378Å
α: 90° β: 90° γ: 120°
R R work R free
0.166 0.164 0.187
Expression system: Escherichia coli BL21