› 5aa2

X-ray diffraction
2.8Å resolution

Crystal structure of MltF from Pseudomonas aeruginosa in complex with NAM-pentapeptide.

Released:
DOI: 10.2210/pdb5aa2/pdb
PDB entry 5aa2 coloured by chain, front view.
PDB entry 5aa2 coloured by chain, side view.
PDB entry 5aa2 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa BWHPSA013
Primary publication:
Activation by Allostery in Cell-Wall Remodeling by a Modular Membrane-Bound Lytic Transglycosylase from Pseudomonas aeruginosa.
Domínguez-Gil T, Lee M, Acebrón-Avalos I, Mahasenan KV, Hesek D, Dik DA, Byun B, Lastochkin E, Fisher JF, Mobashery S, Hermoso JA
Structure 24 1729-1741 (2016)
PMID: 27618662

Function and Biology Details

Reaction catalysed: 
Exolytic cleavage of the (1->4)-beta-glycosidic linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) residues in peptidoglycan, from either the reducing or the non-reducing ends of the peptidoglycan chains, with concomitant formation of a 1,6-anhydrobond in the MurNAc residue.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-219506 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Membrane-bound lytic murein transglycosylase F Chains: A, C
Molecule details ›
Chains: A, C
Length: 499 amino acids
Theoretical weight: 56.26 KDa
Source organism: Pseudomonas aeruginosa BWHPSA013
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HXN1 (Residues: 8-490; Coverage: 100%)
Gene names: PA3764, mltF
Sequence domains:
Structure domains: Periplasmic binding protein-like II
Membrane-bound lytic murein transglycosylase F Chain: B
Molecule details ›
Chain: B
Length: 499 amino acids
Theoretical weight: 56.24 KDa
Source organism: Pseudomonas aeruginosa BWHPSA013
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HXN1 (Residues: 8-490; Coverage: 100%)
Gene names: PA3764, mltF
Sequence domains:
Structure domains: Periplasmic binding protein-like II
Membrane-bound lytic murein transglycosylase F Chain: D
Molecule details ›
Chain: D
Length: 499 amino acids
Theoretical weight: 56.26 KDa
Source organism: Pseudomonas aeruginosa BWHPSA013
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HXN1 (Residues: 8-490; Coverage: 100%)
Gene names: PA3764, mltF
Sequence domains:
Structure domains: Periplasmic binding protein-like II
N-ACETYLGLUCOSAMINE-1,6-ANHYDRO-N-ACETYLMURAMIC ACID L-ALA-D-GLU-M-DAP-D-ALA-D-ALA Chain: E
Molecule details ›
Chain: E
Length: 6 amino acids
Theoretical weight: 790 Da
Source organism: Pseudomonas aeruginosa BWHPSA013

Ligands and Environments

1 bound ligand:
Ligand CL 2 x CL
3 modified residues:
Ligand DGL 1 x DGL
Ligand API 1 x API
Ligand DAL 2 x DAL

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P21
Unit cell:
a: 65.585Å b: 135.652Å c: 137.459Å
α: 90° β: 92.06° γ: 90°
R-values:
R R work R free
0.171 0.167 0.235
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Cell-Wall Recycling of the Gram-Negative Bacteria and the Nexus to Antibiotic Resistance.
Dik et al. (2018)
3 more

2 mentions without citation

Lytic transglycosylases: concinnity in concision of the bacterial cell wall.
Dik et al. (2017)
1 more