5a8c

X-ray diffraction
0.97Å resolution

The ultra high resolution structure of a novel alpha-L-arabinofuranosidase (CtGH43) from Clostridium thermocellum ATCC 27405 with bound trimethyl N-Oxide (TRS)

Released:

Function and Biology Details

Reaction catalysed:
Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbohydrate binding family 6 Chain: A
Molecule details ›
Chain: A
Length: 326 amino acids
Theoretical weight: 36.45 KDa
Source organism: Hungateiclostridium thermocellum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A3DEX4 (Residues: 30-330; Coverage: 46%)
Gene name: Cthe_1271
Sequence domains: Glycosyl hydrolases family 43
Structure domains: Glycosyl hydrolase domain; family 43

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21
Unit cell:
a: 45.44Å b: 67.7Å c: 51.01Å
α: 90° β: 113.09° γ: 90°
R-values:
R R work R free
0.104 0.103 0.124
Expression system: Escherichia coli BL21(DE3)