› 5a7v

X-ray diffraction
1.35Å resolution

The GH130 family of mannoside phosphorylases contains glycoside hydrolases that target beta-1,2 mannosidic linkages in Candida mannan

Released:
DOI: 10.2210/pdb5a7v/pdb
PDB entry 5a7v coloured by chain, front view.
PDB entry 5a7v coloured by chain, side view.
PDB entry 5a7v coloured by chain, top view.
Source organism: Bacteroides thetaiotaomicron
Primary publication:
The GH130 Family of Mannoside Phosphorylases Contains Glycoside Hydrolases That Target β-1,2-Mannosidic Linkages in Candida Mannan.
Cuskin F, Baslé A, Ladevèze S, Day AM, Gilbert HJ, Davies GJ, Potocki-Véronèse G, Lowe EC
J Biol Chem 290 25023-33 (2015)
PMID: 26286752

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183609 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Glycosidase, PH117-related Chain: A
Molecule details ›
Chain: A
Length: 388 amino acids
Theoretical weight: 43.43 KDa
Source organism: Bacteroides thetaiotaomicron
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8A186 (Residues: 20-383; Coverage: 95%)
Gene name: BT_3780
Sequence domains: beta-1,4-mannooligosaccharide phosphorylase
Structure domains: Glycosyl hydrolase domain; family 43
Glycosidase, PH117-related Chain: B
Molecule details ›
Chain: B
Length: 388 amino acids
Theoretical weight: 43.47 KDa
Source organism: Bacteroides thetaiotaomicron
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8A186 (Residues: 20-383; Coverage: 95%)
Gene name: BT_3780
Sequence domains: beta-1,4-mannooligosaccharide phosphorylase
Structure domains: Glycosyl hydrolase domain; family 43

Ligands and Environments

3 bound ligands:
Ligand BMA 2 x BMA
Ligand MAN 4 x MAN
Ligand SO4 10 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Unit cell:
a: 75.767Å b: 118.11Å c: 126.586Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.131 0.13 0.15
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Functions, structures, and applications of cellobiose 2-epimerase and glycoside hydrolase family 130 mannoside phosphorylases.
Saburi W. (2016)
3 more

3 mentions without citation

Glycosyl Oxocarbenium Ions: Structure, Conformation, Reactivity, and Interactions.
Franconetti et al. (2021)
2 more