› 5a24

X-ray diffraction
1.5Å resolution

Crystal structure of Dionain-1, the major endopeptidase in the Venus flytrap digestive juice

Released:
DOI: 10.2210/pdb5a24/pdb
PDB 5a24 coloured by chain and viewed from the front.
PDB 5a24 coloured by chain and viewed from the side.
PDB 5a24 coloured by chain and viewed from the top.
Source organism: Dionaea muscipula
Primary publication:
Enzymatic and structural characterization of the major endopeptidase in the Venus flytrap digestion fluid.
Risor MW, Thomsen LR, Sanggaard KW, Nielsen TA, Thogersen IB, Lukassen MV, Rossen L, Garcia-Ferrer I, Guevara T, Scavenius C, Meinjohanns E, Gomis-Ruth FX, Enghild JJ
J. Biol. Chem. (2015)
PMID: 26627834

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dionain-1 Chain: A
Molecule details ›
Chain: A
Length: 222 amino acids
Theoretical weight: 23.04 KDa
Source organism: Dionaea muscipula
Expression system: Komagataella pastoris
UniProt:
  • Canonical: A0A0E3GLN3 (Residues: 131-352; Coverage: 69%)
Sequence domains: Papain family cysteine protease
Structure domains: Cysteine proteinases

Ligands and Environments

2 bound ligands:
Ligand E64 1 x E64
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2
Unit cell:
a: 73.85Å b: 47.79Å c: 57.79Å
α: 90° β: 92.44° γ: 90°
R-values:
R R work R free
0.158 0.158 0.172
Expression system: Komagataella pastoris

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Citations

6 citation in other articles

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