› 5a10

X-ray diffraction
1.42Å resolution

The crystal structure of Ta-TFP, a thiocyanate-forming protein involved in glucosinolate breakdown (space group C2)

Released:
DOI: 10.2210/pdb5a10/pdb
PDB 5a10 coloured by chain and viewed from the front.
PDB 5a10 coloured by chain and viewed from the side.
PDB 5a10 coloured by chain and viewed from the top.
Source organism: Thlaspi arvense
Primary publication:
The crystal structure of the thiocyanate-forming protein from Thlaspi arvense, a kelch protein involved in glucosinolate breakdown.
Gumz F, Krausze J, Eisenschmidt D, Backenköhler A, Barleben L, Brandt W, Wittstock U
Plant Mol. Biol. (2015)
PMID: 26260516

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thiocyanate forming protein Chain: A
Molecule details ›
Chain: A
Length: 356 amino acids
Theoretical weight: 39.75 KDa
Source organism: Thlaspi arvense
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G1FNI6 (Residues: 1-348; Coverage: 100%)
Sequence domains: Kelch motif

Ligands and Environments

2 bound ligands:
Ligand ACT 1 x ACT
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, DESY BEAMLINE P11
Spacegroup: C2
Unit cell:
a: 119.39Å b: 48.96Å c: 68.27Å
α: 90° β: 109.71° γ: 90°
R-values:
R R work R free
0.158 0.157 0.176
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 citation in other articles

Crystal structure of the Epithiospecifier Protein, ESP from Arabidopsis thaliana provides insights into its product specificity.
Zhang et al. (2016)
2 more

3 mentions without citation

Recognition of the Diglycine C-End Degron by CRL2KLHDC2 Ubiquitin Ligase.
Rusnac et al. (2018)
2 more