› 5a0t

X-ray diffraction
2.28Å resolution

Catalysis and 5' end sensing by ribonuclease RNase J of the metallo- beta-lactamase family

Released:
DOI: 10.2210/pdb5a0t/pdb
PDB entry 5a0t coloured by chain, front view.
PDB entry 5a0t coloured by chain, side view.
PDB entry 5a0t coloured by chain, top view.
Source organisms:
Primary publication:
Linkage of catalysis and 5' end recognition in ribonuclease RNase J.
Pei XY, Bralley P, Jones GH, Luisi BF
Nucleic Acids Res 43 8066-76 (2015)
PMID: 26253740

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131480 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Ribonuclease J Chains: A, B
Molecule details ›
Chains: A, B
Length: 561 amino acids
Theoretical weight: 61.15 KDa
Source organism: Streptomyces coelicolor A3(2)
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O86842 (Residues: 1-561; Coverage: 100%)
Gene names: SC9A10.09, SCO5745, rnj
Sequence domains:
Structure domains:
5'-R(*CP*GP*CP*CP*UP)-3' Chains: E, F
Molecule details ›
Chains: E, F
Length: 6 nucleotides
Theoretical weight: 1.83 KDa
Source organism: Escherichia coli BL21(DE3)

Ligands and Environments

2 bound ligands:
Ligand PEG 2 x PEG
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P4322
Unit cell:
a: 186.187Å b: 186.187Å c: 113.382Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.146 0.145 0.177
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Bacterial ribonucleases and their roles in RNA metabolism.
Bechhofer et al. (2019)
5 more

4 mentions without citation

Targeting Toxoplasma gondii CPSF3 as a new approach to control toxoplasmosis.
Palencia et al. (2017)
3 more