5zr1

Electron Microscopy
3Å resolution

Saccharomyces Cerevisiae Origin Recognition Complex Bound to a 72-bp Origin DNA containing ACS and B1 element

Released:
Related structures: EMD-6941

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
6 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (8 distinct):
Origin recognition complex subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 914 amino acids
Theoretical weight: 104.55 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P54784 (Residues: 1-914; Coverage: 100%)
Gene names: ORC1, YML065W
Sequence domains:
Origin recognition complex subunit 2 Chain: B
Molecule details ›
Chain: B
Length: 620 amino acids
Theoretical weight: 71.34 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P32833 (Residues: 1-620; Coverage: 100%)
Gene names: ORC2, RRR1, SIR5, YBR0523, YBR060C
Sequence domains: Origin recognition complex subunit 2
Origin recognition complex subunit 3 Chain: C
Molecule details ›
Chain: C
Length: 616 amino acids
Theoretical weight: 72.16 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P54790 (Residues: 1-616; Coverage: 100%)
Gene names: L1365, OAF1, OIF1, ORC3, YLL004W
Sequence domains: Origin recognition complex winged helix C-terminal
Origin recognition complex subunit 4 Chain: D
Molecule details ›
Chain: D
Length: 529 amino acids
Theoretical weight: 60.77 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P54791 (Residues: 1-529; Coverage: 100%)
Gene names: ORC4, P9325.5, YPR162C
Sequence domains: Origin recognition complex (ORC) subunit 4 C-terminus
Origin recognition complex subunit 5 Chain: E
Molecule details ›
Chain: E
Length: 479 amino acids
Theoretical weight: 55.35 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P50874 (Residues: 1-479; Coverage: 100%)
Gene names: N0834, ORC5, YNL261W
Sequence domains:
Origin recognition complex subunit 6 Chain: F
Molecule details ›
Chain: F
Length: 435 amino acids
Theoretical weight: 50.37 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P38826 (Residues: 1-435; Coverage: 100%)
Gene names: AAP1, ORC6, YHR118C
Sequence domains: Origin recognition complex subunit 6 (ORC6)
72bp-oring DNA, ACS305, T-rich Chain: G
Molecule details ›
Chain: G
Length: 72 nucleotides
Theoretical weight: 22.2 KDa
72bp-oring DNA, ACS305, A-rich Chain: H
Molecule details ›
Chain: H
Length: 72 nucleotides
Theoretical weight: 22.17 KDa

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution:
Relevant EMDB volumes: EMD-6941
Expression system: Saccharomyces cerevisiae